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Fig. 2 | BMC Medicine

Fig. 2

From: Efficacy of SCF drug conjugate targeting c-KIT in gastrointestinal stromal tumor

Fig. 2

Preparation and bioactivity evaluation of SCF-DM1 A. SDS-PAGE result of SCF and DM1 conjugation product. Lane 1: protein marker; lane 2: SCF and DM1 conjugation product; lane 3: rhSCF. B Membrane KIT expression levels in GIST T1 cells with treatment of drugs. Vertical dot line in B was given to view the induction effect of drugs, and numbers indicated left-side or right-side cell population (%) separated by the dot line in each group. SCF-DM1: 100 ng/mL, rhSCF: 100 ng/mL, DM1: 100 nM. C Immunoblot analysis of phosphorylation status of KIT signaling proteins in THP-1 cells with treatment of drugs. rhSCF: 100 ng/mL; SCF-DM1: 100 ng/mL. D LC–MS result of SCF and DM1 conjugation product. + X DM1 means one molecule of SCF was conjugated with X molecule(s) of DM1, + 1 SMCC means one molecule of SCF was conjugated with 1 molecule of SMCC

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