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Fig. 3 | BMC Medicine

Fig. 3

From: Induction, decay, and determinants of functional antibodies following vaccination with the RTS,S malaria vaccine in young children

Fig. 3

Induction of FcγR-binding antibodies to CSP in younger and older children. Serum samples from children in the RTS,S vaccine group were stratified into younger (12–24 months) and older (24–60 months) age groups from Manhiça (blue box plots; n=11 and n=39, respectively) and Ilha Josina (orange box plots; n=24 and n=26, respectively) study sites. Samples collected after vaccination at month 3 were tested for A FcγRIIa and B FcγRIII-binding to CSP. Boxes represent the interquartile range (IQR) with median (bar) and whiskers represent the highest and lowest values within 1.5× IQR. Y-axis data represent optical density (OD) at 450nm. The percentage of children with a positive response is shown in Additional file 1: Figure S3, and reactivities between unpaired samples were compared using the Kruskal-Wallis rank sum test. For comparisons between Manhica and Ilha Josina: 12–24m age group, p=0.012 for FcγRIIa and p=0.091 for FcγRIII; for the 24–60m age group p>0.05 for both FcγRs. Results for the NANP-repeat and CT regions are shown in Additional file 1: Figure S3. Serum samples from children in the Ilha Josina study site (n=49) were also tested for IgG to the merozoite antigen AMA1 which is an established biomarker of malaria exposure (C, D). Children were classified as having low or high AMA1 IgG based on being below or above the median. Children with low AMA1 IgG had significantly higher FcγR-binding antibodies; Mann-Whitney U-test

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