Fig. 2

Differentially expressed genes (DEG) in AD at bulk and cellular levels. A The Venn diagram shows the overlap of bulk DEGs among four blood transcriptomic datasets. B, C The top enriched biological processes of upregulated (B) and downregulated (C) genes in AD compared with NC in each dataset. The point size represents the number of DE genes in the corresponding pathway. Color represents the significant level (i.e., FDR). D Circos plot shows the overlap between DE genes in each cell type in ADNI, where colors of the outer circle represent cell types. The dark and light oranges in the inner circle indicate genes hitting multiple cell types and only one cell type, respectively. Purple curves link identical genes. E Network of enriched terms in each cell type in ADNI, where terms with a similarity score > 0.3 are connected by edges. The color of the pie chart indicates the cell type which is color-coded as Fig. 2D, where the size of a slice represents the percentage of DEG from each cell type. The node size represents the number of DEG hitting terms