Fig. 2

The inhibitory effect of anidulafungin on different stages of SFTSV infection was evaluated. A Flowcharts based on SFTSV infection assays identified antiviral activity of selected compounds that interfere with the entire stage, virion stability, virus entry, or post-entry stage of SFTSV infection in Vero cells. B The number of intracellular SFTSV RNA copies in Vero cells produced with or without anidulafungin treatment during different stages of the infection assay as measured by qRT-PCR. C Negative-staining EM analyses to visualize SFTSV virions incubated with anidulafungin (20, 50, and 100μM) or vehicle. Bars, 100 nm. D SFTSV protein expression in Vero cells with or without anidulafungin at the different stages was analyzed using Western blot. The significant difference was determined using Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001