Fig. 1

OH2 lysates induce RAW264.7 polarization and phagocytosis in vitro. A Schematic of lysate preparation and flowchart of the experiment performed in the study. B Cell proliferation assay results of CT26 (left), MC38 (middle), and 4T-1 (right) cell lines treated with lysate (red), CFS (blue), and untreated (black) group by CCK8 assay in 24 h. C Flow cytometric analysis results of one representative sample from each cell line with lysate or CFS treatment. D The percentage of M1 (F4/80+CD86+) subtype in the lysate, CFS, and untreated groups of CT26, MC38, and 4T-1 cells detected by flow cytometry. The data are averages from three samples per treatment group. An unpaired Student’s t test was used to analyse the significance of the difference between the two groups and ANOVA was used to analyse the significance of the difference between the groups (>2). E The ratio of M2 (F4/80+CD206+) subtype in the lysate, CFS, and untreated groups of CT26, MC38, and 4T-1 cells detected by flow cytometry. The data are averages from three samples per treatment group. An unpaired Student’s t test was used to analyse the significance of the difference between the groups. F. The phagocytic and killing functions of macrophages treated with different cancer cell lysates detected by CFSE/PI. Three effectors to target ratios were set for each cell line (RAW264.7: tumour cells=25:1, 50:1, and 100:1). The data are averages from three samples per treatment group. Statistical analysis was performed using ANOVA with multiple comparisons. ns, no significant differences, *, p<0.05, **, p<0.01, ***, p<0.001, ****, p<0.0001