Table 1 Flow cytometry qualitative thresholds considered to identify the investigated leukocyte populations

Granulocytes

Neutrophils

SSC^bright, CD45⁺, CD16⁺

Eosinophils

SSC^bright, CD45⁺, CD16^-, CD2 / CD294⁺

Basophils

SSC^int, CD45⁺, CD16^-, CD2 / CD294⁺

Lymphoid lineage cells

Noncytotoxic T lymphocytes

SSC^dim, CD45⁺, CD16^-, CD2 / CD294⁺

Cytotoxic T/NK lymphocytes

SSC^dim, CD45⁺, CD16⁺, CD2 / CD294⁺

B lymphocytes

SSC^dim, CD45⁺, CD16^-, CD2 / CD294^-, CD19⁺

Monocytes

CD16- (classical) monocytes

SSC^int, CD45⁺, CD16^-, CD2 / CD294^-, CD19^-, CD36⁺ CD16^-

CD16+ (non-classical) monocytes

SSC^int, CD45⁺, CD16⁺, CD2 / CD294^-, CD19^-, CD36⁺, CD16⁺

Precursors/Immature cells

Myeloid precursors

SSC^dim, CD45⁺, CD19^-, CD2/ CD294^-

Immature granulocytes

SSC^bright, CD45⁺, CD16^-, CD2 / CD294^-