Fig. 3

Gene expression levels associated with CTG-repeat length. For each gene, a mixed effect model was fitted with before/after CBT and CTG-repeat length as fixed effects, while accounting for (random) effects of the individual. The p-values for the fixed effects were estimated via Satterthwaite’s freedom method and FDR corrected. A Volcano plot of significance (−log10 of the nominal p-value) and effect size of the CTG-repeat length (per 100 CTGs) on gene expression. Genes for which the effect of CTG-repeat is significant (FDR < 0.05) are visualized in black. B For the four genes with the lowest nominal p-values from panel A, the gene expression values (logCPM) are plotted against the CTG-repeat length. Blue dots represent baseline expression values, and red dots expression values after CBT. The regression line is fitted over all values, independent of the time point. The Pearson correlation coefficients for the association between CTG-repeat length and gene expression are displayed for each gene