Fig. 10

C1q neutralization also rescued synaptic elimination, inhibited microglial activation, and ameliorated cognitive dysfunction and anxiety-like behaviors in SIN rats. A The schedule of the fourth experiment. Intracerebroventricular (i.c.v) injection of 10 μl C1q neutralizing antibody or IgG2b in PBS was administered at 1 h after anesthesia end. CFC and OFT were used to estimate the behaviors of rats. B The percentage of freezing time of rats during the CFC test (n = 7 per group). C, D C1q neutralization reduced anxiety-like behaviors of rats in OFT (n = 7 per group). E, F Immunoblotting band of C1qa, iba1, and β-actin. G ELISA showed that C1q neutralization reduced the level of C1q in the hippocampus. H, I C1q neutralization downregulates the C1qa and iba1 expression in the hippocampus. J, K Immunoblotting band of PSD95, SYN1, SYP, and β-actin. L–N C1q neutralization increased the level of PSD95 (L), SYN1 (M), and SYP (N) in the hippocampus. Data was shown as Mean ± SD or median ± IQR, with *P < 0.05 or *P < 0.001; n = 4 per group, Sevo-IgG group vs. Sevo-C1q group