Fig. 3

Selected cells survived by induction of PGC1α-mediated PMCA under glucose deprivation-induced metabolic stress conditions. A, B Immunoblot analysis for PGC1α and PMCA expression in PGC1α knockdown selected cells. Selected cells were transfected with PGC1α siRNA and subjected to glucose deprivation-induced metabolic stress at time series. C, D qRT-PCR for measurement of mRNA expression of PMCA family under glucose deprivation-induced metabolic stress in PGC1α knockdown selected cells. s231, 1-4 and sMCF-7, 1-4; PMCA1-4. E, F Immunoblot analysis of PGC1α and PMCA expression in PGC1α-overexpressed parental cells. Parental cells were overexpressed with PGC1α cDNA and subjected to glucose deprivation-induced metabolic stress at time series. p231, 1-4 and pMCF-7, 1-4; PMCA1-4. O.E-PGC1α; PGC1α-overexpressed. G, H qRT-PCR for measurement of mRNA expression of PMCA family under glucose deprivation-induced metabolic stress in PGC1α-overexpressed parental cells. I, K EMSA of HNF4α and NFκB, its transcriptional coactivator PGC1α with the PMCA1 and 2 promoters. EMSA was performed with nuclear extract (NE) and a [γ-³²P]-labeled oligonucleotide, parental and selected cells. J, L Dual-luciferase reporter assay, which was used to compare HNF4α and NFκB transcriptional activity between parental and selected cells under glucose deprivation-induced metabolic stress in PGC1α knockdown selected cells. Short-term G (−), glucose-deprived conditions for 12 h; long-term G (−), glucose-deprived conditions for 48 h. *P < 0.05 vs. parental or selected, **P < 0.01 vs. selected, **P < 0.01 vs. parental long-term G (−)