Fig. 4

Candidate 13, a novel PMCA inhibitor, in glucose deprivation-induced metabolic stress-resistant cancer cells. A Information about candidate 13. B Measurement of intracellular free calcium (left) and immunoblot analysis for PMCA expression (right) in selected cells after treatment with PMCA inhibitor, caloxin and candidate 13, under glucose deprivation-induced metabolic stress conditions. An arrow indicates the addition of high K⁺ depolarization to induce an increase in Ca²⁺ on traces. C, D Viability assay of the selected cells after treatment with caloxin and candidate 13 with or without glucose deprivation-induced metabolic stress conditions. Data represent the average of at least three separate independent experiments. **P < 0.01 vs. G (−), *P < 0.05 vs. control, **P < 0.01 vs. control. G (+) glucose present, G (−) glucose deprivation