Table 5 Physicochemical property evaluation of vaccine construct
Evaluating parameters | TLR4 adjuvant-vaccine (V1) | TLR3/TLR8 adjuvant-vaccine (V2) |
|---|---|---|
Number of amino acids | 260 | 199 |
Molecular weight | 27,611.20 | 21,730.75 |
Chemical formula | C1273H2001N305O361S8 | C999H1567N261O257S12 |
Theoretical PI | 4.75 | 9.37 |
Total number of atoms | 3948 | 3096 |
Total number of negatively charged residues (Asp + Glu) | 34 | 13 |
Total number of positively charged residues (Arg + Lys) | 23 | 25 |
Extinction coefficient (at 280Â nm in H2O) | 29,700 | 38,555 |
Estimated half-life (mammalian reticulocytes, in vitro) | 30Â h | 30Â h |
Estimated half-life (yeast cells, in vivo) |  > 20 h |  > 20 h |
Estimated half-life (Escherichia coli, in vivo) |  > 10 h |  > 10 h |
Instability index | 30.24 (stable) | 38.12 (stable) |
Aliphatic index | 106.73 | 96.28 |
Grand average of hydropathicity (GRAVY) | 0.418 | 0.292 |
Immunogenicity | 3.87883 | 1.90516 |
Antigenicity (VaxiJen) | 0.5174 (antigen) | 0.6203 (antigen) |
Antigenicity (ANTIGENpro) | 0.209754 | 0.292126 |
Allergenicity (AllerTOP) | Non-allergen | Non-allergen |
Allergenicity (Allergenfp) | Allergen | Non-allergen |
Solubility (SolPro) | 0.965681 (soluble) | 0.601 (soluble) |
Solubility (Protein-Sol) | 0.669 (soluble) | 0.703733Â (soluble) |