Fig. 2

Assessment of blood pressure and liver and kidney function indices in the A2M-overexpression rat model. a Schematic illustration of the establishment of a humanized A2M-overexpression pregnant rat model. b Determination of the A2M levels in the sera of pregnant rats during different stages of gestation (GD7.5 and GD19.5) in the control and A2M-overexpression groups by ELISA. c, d, c1–dI Western blotting data showing the level of A2M in the decidua basalis (c) and feto-placenta (d), and c1–d1 are the quantitative analysis of A2M expression in control and A2M-overexpression rats. e–h The trends in the variation in systolic (e, g) or diastolic (f, h) pressure from the control and A2M-overexpressing groups in both non-pregnant (e, f) and pregnant (g, h) rats. i, j Representative TEM images of the kidneys from both groups. k–o Representative HE staining (k–l) and PAS staining (m, n) of cross-sections of rat kidneys from the control and A2M-overexpression groups, and o shows the quantitative analysis of the area of Bowman’s space from both groups. p Urine protein (mg/24 h) levels at GD7.5 and GD19.5 from the control and A2M-overexpression rats. q–u Determination of the levels of BUN (q), CREA (r), UA (s), ALT (t), and AST (u) in rat sera from the control and A2M-overexpression groups. Scale bars = 5 μm in i, j; 50 μm in k–n. Abbreviations: TEM, transmission electron microscope; Enc, endothelial cell; Podo, podocyte; cap, capillary; BUN, blood urea nitrogen; CREA, creatinine; UA, uric acid; ALT, alanine aminotransferase; AST, aspartate aminotransferase. *P < 0.05, **P < 0.01, ***P < 0.001