Fig. 6

Assessment of feto-placental angiogenesis in the A2M-overexpression rat model. a, a1–a2 Representative PAS and HE staining (a) on the cross-sections of rat placenta from the control and A2M-overexpression groups, and a1–a2 are the quantitative analysis of the area of the placental labyrinthine zone (a1) and the area of placental blood sinusoid (a2) from the control and A2M-overexpression groups. b, b1–b2 Representative immunofluorescent staining of A2M and Caveolin1 in the cross-sections of rat placenta from the control and A2M-overexpression groups (b), and b1–b2 are the quantitative analysis of positive areas of A2M and Caveolin1 expression (%). c–f, c1–f1 Western blotting data showing the expression of A2M (c), Caveolin1 (d), VEGF (e), and CD31 (f) in the placental labyrinthine zone from the control and A2M-overexpression groups. c1–f1 shows the quantitative analysis of A2M (c1), Caveolin1 (d1), VEGF (e1), and CD31 (f1) expression from the control and A2M-overexpression groups. Scale bars = 2 mm in the upper panel of a; 100 μm in the lower panel of a; 50 μm in b. *P < 0.05, **P < 0.01, ***P < 0.001