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Fig. 7 | BMC Medicine

Fig. 7

From: Alpha-2-macroglobulin is involved in the occurrence of early-onset pre-eclampsia via its negative impact on uterine spiral artery remodeling and placental angiogenesis

Fig. 7

Assessing cell migration and tube formation of HUVECs following manipulation of A2M expression. a, a1 Representative images of wound healing assays of HUVECs at 24 h from negative control (control) or A2M-overexpression groups (a), and a1 shows the quantitative analysis of relative cell migration in both groups. b, b1 Representative images of transwell migration assays of HUVECs transfected with either negative control or A2M-overexpression vectors after 24 h of incubation (b), and b1 shows the quantitative analysis of the numbers of migrated cells in both groups. c, c1–c2 Representative fluorescent staining and high magnification images of F-actin on the negative control or A2M-overexpression HUVECs (c), and c1–c2 show the quantitative analysis of the numbers (c1) and length (c2) of filopodia of each cell in both groups. d, d1 Representative immunofluorescent staining of ZO-1 on the negative control or A2M-overexpression HUVECs (d), and d1 shows the quantitative analysis of relative ZO-1 fluorescence intensity (% of control) in both groups. e, e1 Representative images of tube formation assays of HUVECs after 6- and 24-h incubation from negative control or A2M-overexpression groups, and e1 shows the quantitative analysis of total tube length (% of control) at 6-h incubation in both groups. Scale bars = 200 μm in a and e; 50 μm in b and d; 20 μm in c. **P < 0.01, ***P < 0.001

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BMC Medicine

ISSN: 1741-7015

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