Fig. 3

RAGRP3 is a downstream effector molecule of AC092894.1. A Volcano plot of differentially expressed genes obtained by RNA-seq. B KEGG pathway analysis for AC092894.1. The vertical axis represents the pathway. The horizontal axis represents enrichment factors. The size of the dots indicates the number of differentially expressed genes in the corresponding pathway (gene count). The color of the dots indicates the specific value of P-value. C Western blotting to detect the expression of p38 and p-p38 in cell lysates, with α-Tubulin as a control. D RT-qPCR for expression of MAPK signaling pathway-related molecules in LoVo-OxR after overexpression of AC092894.1. E Western blotting to detect the expression of ATF2 and RASGRP3 in cell lysates, with GAPDH was as a control. F Expression of AC092894.1 in our cohort of CRC patients sensitive (n = 28) and resistant (n = 22) to oxaliplatin treatment