Fig. 2

Major biological processes regulated by different TKIs. A The L- or T-shaped designs that span three doses and three time points for each TKI, selected based on results from Fig. 1A–H; 129 samples with three biological replicates per condition were measured in 3’DGE-UMI RNAseq. B Transcriptome changes induced by eight TKIs over dose and time were grouped into 7 clusters based on tSNE analysis, and the corresponding drugs of each cluster were shown in C. D Expression of top 20 gene markers for each cluster. E In Cluster 0, mitochondrial tRNA genes were expressed at a higher level than the other clusters. F Biological processes enriched for gene markers of Cluster 2. Expression of representative genes in GO term of mitotic nuclear division was shown on the right. G Biological processes enriched for gene markers of Cluster 3. Expression of representative genes in this GO endoplasmic reticulum stress was shown on the right. H Biological processes enriched for gene markers of Cluster 4. Expression of representative genes in GO term of heart contraction was shown on the right. I Biological processes enriched for gene markers of Cluster 6. Expression of representative genes in GO term of response to topologically incorrect protein was shown on the right