Fig. 1

GNB4 is upregulated in GC. A GNB4 expression is significantly upregulated in GC tissues (n = 31) compared with that in normal tissues (n = 38) from the GEO dataset. B The mRNA expression levels of GNB4 are examined in the paired tumor and adjacent nontumor tissues (n = 32 pairs) from the GEO dataset. C The mRNA expression of GNB4 is significantly upregulated in tumor tissues of the mouse GC model compare to normal tissues (GSE40634). D GEPIA analysis shows that GNB4 expression is significantly correlated with the clinical staging of patients with GC. E, F Kaplan–Meier plotter analysis reveals that patients with higher GNB4 expression have worse overall survival (OS, E) and progression-free survival (PFS, F) compared with patients with lower GNB4 expression. G Protein expression of GNB4 in GC tissues (T) and adjacent normal tissues (N). H qRT-PCR analysis of GNB4 mRNA expression in paired GC tissues and adjacent normal tissues (n = 10). I, J IHC staining (I) and H-score (J) for GNB4 in adjacent normal tissues (n = 89) and GC tissues (n = 107). Scale bars: 100 μm (inset: 50 μm). K Overall survival was analyzed using Kaplan–Meier curves (log-rank test) in a GC cohort of 101 patients. GC, gastric cancer