Fig. 8

TAS1R3 promotes intestinal inflammation in patients with IBD. a Transcriptome meta-analysis. Only datasets preprocessed and normalized in one step were used (GSE160804/GSE126124/GSE95095/GSE75214/GSE53306). b PCA of intestinal biopsy transcriptomes from patients with and without IBD. Grey and red points represent individual IBD (n = 204) and non-IBD (n = 74) samples, respectively. c Hierarchical clustering and heatmap of up- and downregulated genes. A total of 1,697 significantly DEGs were identified (unpaired Student’s t-test; FDR P < 0.001). d TAS1R3, e MTOR, and f PPARG mRNA expression in intestinal biopsies from patients with (n = 204) and without IBD (n = 74). Spearman’s correlation analysis of associations between intestinal TAS1R3 mRNA expression and that of g MTOR and h PPARG, as well as pro-inflammatory cytokines: i IL1B, j TNFa, and k IL8. All correlations shown are significant. Data are expressed as means ± standard errors of the mean. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (unpaired Student’s t-test). DEF, differentially expressed gene; FDR, false discovery rate; IBD, inflammatory bowel disease; PCA, principal coordinate analysis