Fig. 2

Adipose tissue-specific Adipsin overexpression improves microvascular dysfunction. A Representative Western blot images of Adipsin levels in serum and heart from different groups. B Quantitative analysis of serum Adipsin levels in A. C Quantitative analysis of Adipsin levels in cardiac tissues in A. D Representative immunofluorescence images of Adipsin around blood vessels. Scale bar = 20 μm. E Quantitative analysis of Adipsin fluorescence intensity in D. F Representative scanning electron micrographs of cardiac microvessels corrosion in different groups. Insert boxes indicated areas of magnification shown below. Scale bar = 10 μm (upper) and 5 μm (bottom). G Representative transmission electron micrographs of cardiac capillaries with lanthanum nitrate staining indicating barrier function. Insert boxes indicated areas of magnification shown below, and red arrowheads indicated areas of lanthanum nitrate deposition. Scale bar = 1 μm (upper) and 1 μm (bottom). H Representative immunofluorescence images of CD31 in cardiac tissue. Scale bar = 20 μm. I Quantitative analysis of blood vessel density in H. Data were presented as mean ± SEM. One-way ANOVA was used for statistical analysis