Fig. 8

Adipsin directly bounds to Csk, impeding Src activity and VE-cadherin internalization. A Venn diagram showed the protein candidates as the targets of Adipsin. B GO analysis of Adipisn-associated proteins. GO, Gene Ontology; BP, biological process; CC, cellular component; MF, molecular function. C Representative Western blot images for co-immunoprecipitation of Adipsin interacting with Csk. D Representative immunofluorescence images of cell-cell junctions, and white arrowheads indicated endothelial cell junctions. Scale bar = 50 μm. E Quantitative analysis of FITC-conjugated Dextran intensity evaluating monolayer cellular permeability. F Measurements of trans-endothelial electrical resistance (TEER). G Representative Western blot images for VE-cadherin (total), VE-cadherin (membrane), phospho-VE-cadherin (Tyr685), phospho-VE-cadherin (Tyr731), Src, and phospho-Src (Tyr416). H–K Quantitative analysis of Western blot images from three independent experiments in G. Data were presented as mean ± SEM. One-way ANOVA was used for statistical analysis