Fig. 2

PTPN22 mediates OM-induced osteogenic responses in vitro. A-B AVICs were transfected with the PTPN22 expression vector (pCMV3-PTPN22) or empty vector ((pCMV3-control). The immunoblotting results show the expression of ALP and Runx2 in vitro. n = 3. C-D Cells were treated with PTPN22 siRNA following stimulation with OM. Representative western blot and quantitative data on the relative abundance of ALP, Runx2 and BMP2 in the different groups. Cells were transfected with pCMV3-PTPN22. Images of DCFH-DA staining E and TMRM staining (F). n = 4. Scale bar = 25 μm. AVICs were transfected with PTPN22 siRNA. Images of DCFH-DA staining (G) and TMRM staining (H). n = 4. Scale bar = 25 μm. I-J Western blot analysis shows the levels of NF-κB and ERK1/2 phosphorylation after PTPN22 knockdown. n = 4. All data are shown as the means ± SEM. *p < 0.05; **p < 0.01