Fig. 3

PTPN22 deletion mitigates aortic valve lesions in vivo. Echocardiographic data in a wire injury-induced CAVD mouse model. A-B Peak transvalvular jet velocity, aortic valve peak pressure, and AVA. C Image of aortic valves in the aortic valve calcification model. H&E staining of aortic valves after wire injury. Scale bar = 200 μm. D Masson’s trichrome staining. Scale bar = 50 μm. E Von Kossa staining. Scale bar = 50 μm. F-G Immunofluorescence images of the aortic valve (DAPI: blue, WGA: green). Scale bar = 50 μm. n = 5. Values are the means ± SEMs. #p < 0.05, ##p < 0.01 vs. sham group; *p < 0.05, **p < 0.01 vs. AVI + PTPN22^{−/− group}