Fig. 4
Discovery and characterization of compound S18, a novel inhibitor of PTPN22. A The molecular structure of S18. B The binding affinities of S18/PTPN22 (Kd = 86.8 μM) and S18/GFP control (Kd = no binding) were detected by MST assay. Values are the means ± SEM. n = 3. *p < 0.05. The detailed docking mode of (C) N75/PTPN22 and (D) S18/PTPN22 with docking pock. E Cell viability of human AVICs treated with S18 for 3 days. n = 5. F Cell morphology of human AVICs treated with S18 for 3 days. Scale bar = 25 μm. G-H Alizarin Red S staining of cells cultured under different conditions. n = 4. Values are the means ± SEM. #p < 0.05 vs. the control group, *p < 0.05 vs. the OM group