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Fig. 5 | BMC Medicine

Fig. 5

From: Targeted inhibition of PTPN22 is a novel approach to alleviate osteogenic responses in aortic valve interstitial cells and aortic valve lesions in mice

Fig. 5

Piericidin diglycoside S18 exhibited a remarkable anti-osteogenic responses effect. Human AVICs were cultured with OM and treated with or without 0.5 µM S18 for 3 days. A-C Western blotting and quantification analysis showed the expression of ALP, Runx2 and BMP2 in human AVICs. n = 4. D-G Immunofluorescence staining of ALP (green), Runx2 (red) and BMP2 (green) in human AVICs (DAPI: blue). n = 5. Scale bar = 100 μm. H qRT-PCR was employed to measure ALP mRNA and Runx2 mRNA expression. n = 4. Values are the means ± SEM. #p < 0.05 vs. the control group, *p < 0.05 vs. the OM group

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BMC Medicine

ISSN: 1741-7015

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