Fig. 1

Figure to show summary of IMCY-0098 structure and the HLA class II epitopes it will bind based on previous literature [27] (A), in vitro thioredox data (B), in vitro binding data for DR3 (C), and DR4 (D). IMCY-009 sequence is shown as it overlaps with proinsulin (A). The design was based on previous literature [27]. Thiol-oxidoreductase activity was assessed in vitro on a disulfide-linked tripeptide substrate wherein a fluorescent signal was generated after disulfide bridge reduction using Sensolyte^® 520 Thiol Quantification kit. Results are expressed as a relative activity percentage compared to the dithiothreitol (DTT) after 45 min of incubation with the Thiol detection reagent (B). Binding affinities with DR3 and DR4 (C and D, respectively) were confirmed in a classical competition assay with a reference peptide (a high-affinity canonical 1–14 epitope derived from the YAR-B antigen), in which proinsulin-derived high-affinity binders would be identified