Fig. 3

Cannabinoid receptors expression in human fetal testis. (A) RNA-seq quantification of transcripts coding for CB1 (CNR1) and CB2 (CNR2) in bulk embryonic human testis from 6 to 17 DW. Transcripts amount is expressed in Fragments Per Kilobase Million (FPKM). (B) Expression profiles of transcript isoforms coding for CB1, CB1A, CB1B, and CB2A. RT-qPCR was performed on testes from 3 human embryos at 7 DW. Histograms represent mean expression (relative to RPLP0) ± SEM. (C) Detection of CNR1 and CNR2 transcripts by RNAscope (red dots, some pointed by white arrows) on 10–12 DW human testis section co-immunostained with specific antibodies for Leydig cells (CYP11A1-positive cells), germ cells (LIN28-positive cells), and Sertoli cells (AMH-positive cells) in green. The right panel of each probe corresponds to an higher magnification. Scale bar = 70 and 30 µm for the higher magnification. The detection of dihydrodipicolinate reductase (dapB) and polymerase II transcripts was used as negative and positive control respectively on 10–12 DW human testis section co-immunostained with specific antibodies for Sertoli cells