Fig. 6
Effect of CBD and/or THC on Sertoli cells functions. (A) Amount of AMH in the supernatants of fetal human testis explants of 8–10 or 10–12 DW cultured for 72 h in presence of solvent controls (DMSO and/or EtOH) or 10−5 M of CBD and/or THC. Results are expressed related to the amount of AMH produced during the first day of the culture (D0) and as fold from the respective control. Data are means + / − SEM. Control and treated conditions were compared two by two using Wilcoxon tests (*, p < 0.05; **, p < 0.01). (B) Quantification of the surface area occupied by AMH-positive cells (AMH +) after CBD and/or THC treatment at 10−5 M compared to respective control in 8–10 and 10–12 DW embryonic testis. Representative immunostaining of AMH, a specific marker of Sertoli cell, in explants of an 8–10 DW human fetal testis treated with (C) DMSO/EtOH (control) or (D) CBD/THC 10−5 M. AMH appears dark brown (DAB staining) in intracordonal tissue, and sections were counterstained with hematoxylin (in blue). Scale bar = 100 µm