Fig. 7
Effect of CBD and THC alone or in mixture on the transcriptome of human fetal testis. (A) Analysis of differentially expressed genes (DEG) in 8–10 and 10–12 DW embryonic testis after 72 h exposure to CBD, THC, or CBD/THC at 10−5 M, compared to controls. n = 10 for each condition. Data statistical analysis shows 187 DEGs in total, either upregulated (red triangle) or downregulated (blue triangle). These DEGs are distributed in 9 patterns (P) of expression, from P1 to P9. (B) Heatmap representation of transcriptional changes in 8–10 and 10–12 DW embryonic human testis after 72 h exposure to CBD, THC, or CBD/THC at 10−5 M, compared to controls. Heatmap represents the 9 patterns of gene either depleted (down regulated in blue) or enriched (up regulated in red). Genes belonging to enriched pathway are found in P1, P2, and P5 indicated on the right column of the panel. The number of genes belonging to the pathways and p-value for each pathway is indicated in bracket. (C) Schematic representation of the cholesterol biosynthesis pathway, with genes encoding enzymes indicated in blue. Red arrows indicate the significant decrease of gene expression in 8–10 or 10–12 DW embryonic testis after CBD and/or THC treatment compared to control condition. All of them are deregulated after CBD treatment of 10–12 DW testis