Expression of the endocannabinoid system and response to cannabinoid components by the human fetal testis

Table 1 Primers used for qPCR experiments to characterize isoforms of endocannabinoid receptors

Target	Primer sequence (5′-3′)	Amplicon size	Annealing temperature
CNR1 (CB1)	F: TCAGTACGAAGACATCAAAGGTG	85pb	60 °C
CNR1 (CB1)	R: CTTCCCCTAAAGGAAGTTAAAGG	85pb	60 °C
CB1A	F: AGACATCAAAGGAGAATGAGGAG	113pb	60 °C
CB1A	F: AGACATCAAAGGAGAATGAGGAG	113pb	60 °C
CB1B	F: AGACATCAAAGGAGAATGAGGAG	84pb	64 °C
CB1B	R: AATGTTCACCTGGTCTGCTG	84pb	64 °C
CB2A	F: GATTATGCCAGCCAGATGC	77pb	64 °C
CB2A	R: GCTCGGTGAGTGAGAGGTG	77pb	64 °C

ISSN: 1741-7015