Fig. 2

Evaluation of the wound healing and proliferation-regulatory effects of hucMSCs on chondrocytes before and after SF stimulation. A Wound healing of chondrocytes with hucMSCs and/or SF treatment at 0 h, 24 h, and 48 h. Scale bars = 200 μm. B Quantification of the wound area ratio (blank area/total area) at 0 h, 24 h, and 48 h. C Quantification of PCNA-expressed cell ratio (positive cells/total cells). D Cell immunofluorescence of chondrocytes with hucMSCs and/or SF treatment at 24 h, n = 3. E Expressions of anabolic and catabolic genes of chondrocytes. F Protein expression of MMP13 in chondrocytes. Values are presented as mean ± SD. The different letter symbols (a, ab, b, c, and d) indicate significant difference between each other (Fisher's LSD, P < 0.05 or P < 0.01) in descending order of data from a to z. Although the overlapped letters (a versus ab and ab versus b) were statistically different, their difference between each other was not significant. All experiments were repeated at least three times