Fig. 4

Evaluation of the anabolic and catabolic effects of MSC^OE and MSC^KD on chondrocytes by qRT-PCR and WB. A mRNA expression of Mmp13 and Col2 in chondrocytes treated with normal hucMSCs and MMP13-overexpressing hucMSCs. B Protein expression levels of MMP13, Adamts4, and Col2 in chondrocytes treated with normal hucMSCs or MMP13-overexpressing hucMSCs. C mRNA expression levels of Col2, Col10, Adamts4, and Adamts5 in chondrocytes treated with normal hucMSCs, normal hucMSCs in the presence of SF and MMP13-knockdown hucMSCs in the presence of SF. D Protein expression levels of Col2 and Col10 in chondrocytes treated with hucMSCs, hucMSCs in the presence of SF, and hucMSCs with MMP13 knockdown in the presence of SF. MSC^NC: nontargeting control siRNA-treated hucMSCs, MSC^KD: MMP13-knockdown siRNA-treated hucMSCs. The medium of MSC^NC group was replaced by IMDM containing 10% FBS (v/v) and 10% PBS (v/v), and the medium of MSC^NC + SF group and MSC^KD + SF groups were replaced by IMDM containing 10% FBS (v/v) and 10% SF (v/v). Values are presented as mean ± SD. ^#P < 0.05 versus MSC^NC group; ^##P < 0.01 versus MSC^NC group; ^**P < 0.01 versus MSC^NC + SF group. All experiments were repeated at least three times