Fig. 3

Overexpression of NISCH effects mouse behavior, and antihypertensive drugs rescued mice’s spatial working memory by affecting the NISCH expression. A Diagram of stereoscopic injection location and fluorescence expression in mouse brain. Scale bars represent 100 μm. B Open field test of NISCH OE mice and control mice. Measurement of total distance traveled, time in the center and distance in the center of the 5 min test. Data are shown as mean ± SD. n = 18 mice per genotype. C Elevated plus maze test of NISCH OE mice and control mice. Measurement of total distance traveled, time in the open arm, and distance in the open arm of the 5 min test. Data are shown as mean ± SD. n = 18 mice per genotype. D The spontaneous alternation score from the Y-maze test of NISCH OE mice and control mice and the Y-maze test of NISCH OE mice treated with clonidine for 3 days and control mice treated with DMSO for 3 days. Data are shown as mean ± SD. n = 18 mice per genotype. E U251 cells were treated with 0.1 mM clonidine or 0.1 mM tizanidine for 72 h to detect the effect of antihypertensive drugs on NISCH expression. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001. NISCH OE, NISCH overexpressed