Fig. 4

CUS and CSF1Ri inhibited Csf1r and angiogenic genes in mice. (A) GO-BP enrichment analysis shows top-ranked pathways of DEGs derived from group comparisons. (B) Volcano plot shows these DEGs, which are colored in red if -Log₁₀ adj. p ≥ 1.3 and |Log₂FC| ≥ 0.2 and in blue if |Log₂FC| < 0.2. Angiogenic DEGs are highlighted by red dots with gene symbols when -Log₁₀ adj. p ≥ 3. (C) Heatmap shows 38 angiogenic DEGs. Downregulated (in purple frame) and upregulated (in orange frame) genes are highlighted in the heatmap. (D) mRNA expression of Csf1r and (E) mRNA expression of Pik3cg in the PFC (n = 7 mice per group). Ctr: Control; CUS: chronic unpredictable stress; CSF1Ri: CSF1R inhibitor; Veh: Vehicle. Data presented as mean ± SEM; **/*** p < 0.01/0.001 compared to Ctr-Veh. Two-way ANOVA with Bonferroni’s correction. See also Additional file 1: Tables S4 & S5 and Additional file 2: Fig. S2