Fig. 2

Classification of T and B cells in breast cancer into different subpopulations. A The t-SNE plot of T cells. B Feature plots showing the expression of key genes in T cells. The colour scale represents the SAVER imputed gene expression value (log10 scale) for each cell. C Representative images of breast cancer sections stained by fluorescent multiplex immunohistochemistry showing the expression of CD103, LAG3, and CD8 in T cells. D The t-SNE plot of B cells. E The t-SNE plots showing the average expression of BRM marker genes. F Representative images of breast cancer sections stained by fluorescent multiplex immunohistochemistry showing the expression of CD103, LAG3, and CD79A in B cells. Scale bar, 50 μm