Fig. 2

TMIGD1 expression is decreased in the intestinal mucosa of patients with CD and mice with chemically induced colitis. A TMIGD1 mRNA expression in intestinal mucosa in the in-house dataset (NC, n=10; CD, n=7; left) and in the 1000IBD cohort (NC, n=107; CD, n=64; right). B The expression of TMIGD1 mRNA in human intestinal mucosa was assessed via qPCR (NC, n=55; CD, n=64). C The level of TMIGD1 protein in human colonic mucosa. D UMAP plot displaying single cells, which are colored as per shared nearest neighboring clusters and cell types based on single-cell sequencing of human colonic biopsy samples (n=9) from the GSE116222 dataset (left). The feature plot demonstrates TMIGD1 expression, with each dot representing a single cell (middle). Relative mRNA expression of TMIGD1 in different cell types (right). E Representative IHC images of TMIGD1-stained colon sections of NC and different stages of CD. Scale bars, 200 μm (top) and 50 μm (bottom). F The staining intensity of TMIGD1-stained colon sections (NC, n=13; CDAI<150, n=10; 150≤CDAI<220, n=12; 220≤CDAI<450, n=15; CDAI≥450, n=5). The staining intensity of the positive area was quantified using ImageJ software. G Spearman’s correlation analysis between the staining intensity of TMIGD1 and CRP, SES-CD, and GHAS (n=42). H The expression of Tmigd1 mRNA in DSS-induced (water, n=8 and DSS, n=10) and TNBS-induced (Alcohol, n=10; TNBS, n=10) colitis was examined via qPCR. I The expression of Tmigd1 protein in DSS-induced (up) and TNBS-induced (down) colitis. J The expression of Tmigd1 protein in DSS-induced (up) and TNBS-induced (down) colitis was measured via IHC. Scale bars, 100 μm. Data are expressed as mean ± SEM. * p<0.05, *** p<0.001