Fig. 6

BANF1 is crucial for TMIGD1 to maintain the intestinal epithelial barrier and attenuate inflammation via the NF-κB pathway. A The expression level of BANF1 protein in the colonic tissues of patients with CD and healthy individuals. B Degradation of BANF1 protein in NCM460 cells treated with 50 μg/mL CHX was evaluated. C, D TEER and FD4 permeability were measured after TNF-α stimulation in Caco2 monolayer model. E, F The expression levels of AJC proteins, p65, and phosphorylated p65 after TNF-α stimulation in NCM460 cells. G, H TEER and FD4 permeability were measured after TNF-α stimulation in Caco2 monolayer model. I, J Immunoprecipitate by anti-BANF1 antibody (I) and anti-p65 antibody (J) from the lysates of NCM460 cells. IP lysate (10%) was used as input. K Confocal microscope showing colocalization of BANF1 and p65 in NCM460 cells. Scale bars, 10 μm. L, M After TNF-α stimulation, expression of p65 and BANF1 in cytoplasmic and nuclear fractions (left), transcription factor binding assay of nuclear p65 (middle), and subcellular localization of p65 (right) in NCM460 cells. Scale bars, 10 μm. Data are expressed as mean ± SEM. ** p<0.01, *** p<0.001