Fig. 8

TMIGD1 expression predicts response to anti-TNF treatment. A, B Heatmap and box plot from GSE111761 showing different baseline expressions of mRNA in LPMCs of 3 anti-TNF responding patients with CD and 3 non-responders. C, D Heatmap and box plot of the GSE134881 dataset showing different baseline expressions of mRNA in intestinal tissues of anti-TNF responding (n=24) and non-responding (n=36) patients with CD. E Receiver operating characteristic (ROC) curve for the prediction of anti-TNF response with baseline TMIGD1 mRNA expression (AUC=0.745, 95% CI: 0.540–0.950). F The expression of TMIGD1 protein in colonic tissues before anti-TNF treatment. G Representative IHC images of TMIGD1-stained sections from responders and non-responders before anti-TNF treatment. Scale bars, 200 μm (top) and 50 μm (bottom). H The staining intensity of TMIGD1-stained sections from responders (n=10) and non-responders (n=10). The staining intensity of the positive area was quantified using the ImageJ software. Data are expressed as mean ± SEM. * p<0.05, *** p<0.001