Fig. 2

The overexpression of CLU in CCA. A Immunoblotting analysis of CLU in bile from 8 CCA patients and 8 benign biliary diseases patients. B Representative immunohistochemistry images and the level of CLU expression in CCA tissues and interlobular bile duct tissues (normal); the red arrow points to the interlobular bile duct. C, D The overall survival (OS) and relapse-free survival (RFS) curves of CLU in CCA; the blue represents low expression, and the orange represents high expression. E, F Immunoblotting analysis of CLU in cell and cell supernatant from four CCA cell lines and HIBEpiC cell line. G The mRNA level of CLU in four CCA cell lines and HIBEpiC cell line. H, I Immunoblotting analysis of CLU in cell and cell supernatant from five primary CCA cells and HIBEpiC cell. J The mRNA level of CLU in five primary CCA cells and HIBEpiC cell. *p < 0.05, **p < 0.01, ***p < 0.001