Fig. 4

Norharman targeted Raftlin 1 (Rftn1) to restrain the M1 polarization of macrophages. A Heatmap of RNA sequences (n = 3). B Validation of DEGs in vitro by qPCR (n = 3). C, D Norharman inhibited M1 macrophage activation after Rftn1 knockdown by qPCR (n = 4). E, F Norharman reversed the high ratio of M1 macrophages induced by Rftn1 knockdown through flow cytometric analysis (n = 3). G, H Norharman upregulated the mRNA expression of Rftn1 and M1 macrophage markers in pancreatic and intestinal tissues of the mice with AP by qPCR and western blot (n = 4). I, J Norharman upregulated the protein expression of Rftn1 in pancreatic and intestinal tissues of the mice with AP by western blot (n = 3). Data are presented as the mean ± SEM; *P < 0.05 and **P < 0.01 vs. the NC or Ctrl group; ^#P < 0.05 and ^##P < 0.01 vs. the siRftn1 or shRftn1 or AP group