Fig. 8

Rftn1 knockout counteracted the protective effect of norharman on mice with AP. A Schematic diagram of Rftn1 knockout mice with the genotype Rftn1[flox/flox, lyz2-cre]. B By detecting the concentrations of AMS in serum, the level of serum amylase was upregulated in the Rftn1^−/−  + AP group but unchanged in the Rftn1^−/−  + AP + Nor group (n = 6). (C) Severe pathological changes of pancreas in Rftn1^−/− + AP mice compared with that in AP mice (n = 5). (D-E) Compared with AP group, elevated M1 macrophage ratio was found in the spleen from Rftn1^−/− + AP mice, but without change after norharman treatment by flow cytometry analysis (n = 3). F, G Through qPCR and agarose gel electrophoresis, the effect of norharman promoting Rftn1 expression disappeared after substitution the promoter by infection with the Rftn1 lentiviral vector (n = 5). H Alteration of the lipid rafts structure after Rftn1 knockout and overexpression by laser confocal microscopy (1000 ×). Data are presented as the mean ± SEM; **P < 0.01 vs. the Ctrl group or the WT group; ^##P < 0.01 vs. the AP group or the Rftn1^−/− group; ^&&P < 0.01 vs. the Rftn1^−/−  + NC group