Maternal pre-pregnancy BMI, offspring epigenome-wide DNA methylation, and childhood obesity: findings from the Boston Birth Cohort

Table 3 Mediation effect of CpG sites on the maternal BMI-child overweight or obesity (OWO) association in 903 mother–child pairs and 739 non-smoking during pregnancy mother–child pairs from the Boston Birth Cohort

CpG	Gene	Outcome	Whole sample (N = 903)		Subset without maternal smoking (N = 739)
CpG	Gene	Outcome	% Mediated	P	% Mediated	P
cg06292624	AKAP7	OWO at 13 years	3.99	0.011	3.18	0.048
		OWO at 9 years	4.38	0.007	3.52	0.047
		OWO at 8 years	5.00	0.004	3.91	0.044
		OWO at 7 years	5.04	0.007	4.40	0.038
cg17886022	LINC00174	OWO at 13 years	8.78	0.005	9.95	0.033
		OWO at 9 years	9.36	0.002	10.71	0.019
		OWO at 8 years	9.17	0.005	11.29	0.015
		OWO at 7 years	8.98	0.010	11.53	0.022
cg04347874	*NKX2-1*	OWO at 9 years	8.58	0.005	0.43	0.610
		OWO at 8 years	8.17	0.011	0.35	0.630
		OWO at 7 years	8.94	0.005	0.60	0.600
cg04390217	PECR	OWO at 13 years	5.95	0.006	2.60	0.080
		OWO at 9 years	5.14	0.011	2.10	0.100
		OWO at 8 years	5.30	0.013	2.01	0.120
cg09545197	*KCNA3*	OWO at 8 years	13.35	0.016	3.13	0.180
		OWO at 7 years	15.83	0.007	3.89	0.170
cg13331383	TMEM203	OWO at 7 years	5.66	0.016	8.68	0.036
	NDOR1	OWO at 5 years	8.22	< 0.001	11.53	0.001
cg04383707	NCCRP1	OWO at 13 years	4.67	0.006	2.14	0.110
cg08391482	*SERBP1*	OWO at 13 years	10.57	0.010	1.25	0.250
cg01886035	CGREF1	OWO at 9 years	4.32	0.009	1.07	0.320
cg14015044	TNFRSF10C	OWO at 8 years	25.21	0.010	30.75	0.010
cg23732384	MAP2K7	OWO at 7 years	10.19	0.016	15.80	0.003
cg02059896	(*PIKFYVE*^a)	OWO at 3 years	21.69	< 0.001	1.64	0.490
cg14257335	KLHL22	OWO at 3 years	17.41	0.004	19.79	0.002
cg15059222	*CAMK2B*	OWO at 3 years	14.88	0.003	2.80	0.150

All models adjusted for maternal age, education level, race, parity, smoking (only in analysis among whole mother–child pairs), alcohol consumption, gestational age, child sex, birthweight, cord blood cell compositions (CD8, CD4, N.K., B cell, monocytes, granulocytes, nucleated red blood cells), and all surrogate variables
^aFor inter-genic CpG sites, we used the UCSC Genome Browser on Human (GRCh37/hg19) to locate the nearest annotated gene

ISSN: 1741-7015