Fig. 2

Construction of CD133 CAR-T and PD-1 s cells based on sleeping beauty transposons from minicircles. A Schematic diagram of CAR-T cell preparation and sequences of SB100, CD133 CAR-T and CD133 CAR-T and PD-1 s cells. B The minicircles from CD133 CAR, CD133 and PD-1 s CAR and SB100 cells were evaluated by agarose gel electrophoresis. C Representative flow cytometry analyses of CAR-T cell expression using a c-Myc-tag as shown in the surrogate. D The cell viability of CAR-T cells from 3 different donors. E CAR expression detected at different time points from 3 different healthy donors. One colour represents one patient sample. F Western blot detection of the secreted PD-1 blocking scFv in the supernatant of Mock T cells, CD133 CAR-T cells and CD133 CAR-T and PD-1 s cells