Fig. 2

Improvement of liver dysfunction following ApoA-I-complexes (CER-001) infusions. A Top panel shows representative H&E staining of hepatic tissue of LPS and treated groups (n = 6 independent pigs per group). Liver sections (upper panel, scale bar = 400 μm; middle panel, scale bar = 200 μm; lower panel, scale bar = 80 μm). Hepatic injury was defined as the amount of destruction of hepatic lobules (black arrow) and infiltration of inflammatory cells (black dashed arrow). B Liver injury was calculated from whole images of stained liver, as calculated from five randomly selected fields per sample (n = 6 independent pigs per group) and scored from 1 through 4 according to % area of involvement per HPF. Results are presented as mean ± SEM. Significant differences were assessed by one-way ANOVA with Tukey correction (*p < 0.05, **p < 0.005, ***p < 0.0005). C Serum levels of ALT enzyme were measured by ELISA assay (n = 6 independent samples per time point and group). The gray bands show two infusions (0–1 h and 3–4 h) of saline or CER-001 with flow rates 250 ml/h. Results are presented as mean ± SEM. Significant differences were assessed using a two-way ANOVA for repeated measures with Tukey correction (n.s.: p > 0.05, *p < 0.05, **p < 0.005, ***p < 0.0005 vs the LPS group; §p < 0.05; §§p < 0.005, §§§p < 0.0005 vs the CER20 group). Details of differences between corrected and uncorrected comparisons are listed in Additional File 1: Table S1