Fig. 3

Improvement of renal dysfunction following ApoA-I-complexes (CER-001) infusions. A–F sCr, sCystatin C, sKIM-1, UCr, U-Cys, and U-KIM-1 levels were measured by ELISA assay (n = 6 independent samples per time point and group); urinary output (ml/kg/h) was recorded for each animal. The gray bands show two infusions (0–1 h and 3–4 h) of saline or CER-001 with flow rates 250 ml/h. Results are presented as mean ± SEM. Significant differences were assessed using a two-way ANOVA for repeated measures with Tukey correction (n.s.: p > 0.05, *p < 0.05, **p < 0.005, ***p < 0.0005 vs the LPS group; §p < 0.05; §§p < 0.005, §§§p < 0.0005 vs the CER20 group). G, H & E staining was performed on paraffin kidney sections. Renal sections (upper panel, scale bar = 400 μm; middle panel, scale bar = 200 μm; lower panel, scale bar = 80 μm). The kidney desquamation and vacuolization of proximal tubular epithelial cells (zoomed image, 80 µm, black dashed arrow), fibrin deposition and loss of capillaries in numerous glomeruli, Bowman’s capsule expansion (zoomed image, 80 μm, black arrow), and interstitial inflammatory infiltrate (zoomed image, 80 μm, blue arrow) were observed by H&E staining. H, I Tubular and glomerular pathological score was obtained as described in the “Methods” section (n = 6 for each group). Results are presented as mean ± SEM. Significant differences were assessed by one-way ANOVA with Tukey correction (*p < 0.05 **p < 0.005, ***p < 0.0005). Details of differences between corrected and uncorrected comparisons are listed in Additional File 1: Table S1