Fig. 4

Excretion of LPS into the bile via CER-001 transport. A Serum levels of LPS were measured by ELISA assay (n = 6 independent samples per time point and group). B Representative western blot and densitometric analysis of LPS and β-actin protein expression (n = 6 per group). C Endotoxin levels in bile samples were dosed by ELISA assay. D Human ApoA-I levels in bile samples were evaluated by ELISA assay. E The serum human ApoA-I for CER-001 was determined at different time points by ELISA assay. The gray bands show two infusions (0–1 h and 3–4 h) of saline or CER-001 with flow rates 250 ml/h. The data are presented as the mean ± SEM. Statistically significant differences were assessed by one-way ANOVA with Tukey correction (*p < 0.05, **p < 0.005, ***p < 0.0005 vs the LPS group; §p < 0.05; §§p < 0.005, §§§p < 0.0005 vs the CER20 group)