Fig. 3

High-resolution gene expression atlas of the TAD tissue by Tomo-seq. A We obtained the TAD tissue and cut it perpendicular to the tear to approximately 4 mm wide and 1 cm long as the region of interest. And the schematic diagram of Tomo-seq. B Heatmap showing the pairwise correlation of gene expression profiles between sections. Red indicated a high correlation, and blue indicated a low correlation. C Dendrogram showing the hierarchical clustering of sections by gene expression profiles. Sections AD1 ~ AD4 showed a clear separation from other sections. D Heatmap showing the spatially restricted expression of genes that were highly expressed in at least 4 consecutive sections. Rows were genes, and columns were sections. Yellow indicates high expression, and purple indicated low expression. E Heatmap showing the pairwise correlation of expression pattern in sections between identified locally expressed genes. Red indicates a high correlation, and blue indicates a low correlation. The left bar shows the beginning of the high-expression peak among sections. Cluster I–IV indicates hierarchical clustering of expression traces for all genes that were found to be differentially expressed in D