Fig. 5
Validation of the trend of NINJ1 identified in cluster I. A Immunostaining for NINJ1 co-localized with TPPP3 in the human TAD tissue. B Representative images of immunostaining for NINJ1 in the TA and the RA, and quantification of the NINJ1 positive area (n = 8/group). Scale bar indicates 100 μm. C Validate high expression of NINJ1 in TA by qPCR (n = 8/group). D Validate high expression of NINJ1 in TA by WB. E Multilabel immunofluorescence showed NINJ1 was co-localized with cell death marker Tunel and GSDMD, and inflammation marker HMGB1, both in the neointima and media. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; quantitative analysis of NINJ1 positive area was calculated as the ratio of positive area/total area of tissue section; quantitative data were shown as mean ± SEM; statistical analysis was performed with Student’s t test (C, B, D)