Fig. 6

Multiple immunofluorescence detection on the NINJ1-expressing cells. A Immunofluorescent staining of CD3, GSDMD, HMGB1, and NINJ1 in TA and RA. B The quantitative analysis of the expression proportion of NINJ1 in CD3⁺ T cells between TA and RA. C Immunofluorescent staining of CD11b, GSDMD, HMGB1, and NINJ1 in TA and RA. D The quantitative analysis of the expression proportion of NINJ1 in CD11b⁺ macrophages between TA and RA. E Immunofluorescent staining of NINJ1, CD31, and α-SMA in TA and RA. F The quantitative analysis of the number of NINJ1⁺CD31⁺α-SMA⁺ cells per mm² between TA and RA. In B, D and F, n = 8 for each group; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; quantitative analysis of NINJ1 positive cells was calculated as the ratio of the number of NINJ1 positive points in these cells /a total number of CD3⁺/CD11b⁺/ CD31⁺α-SMA.⁺ cells in the tissue section; quantitative data were shown as mean ± SEM; Statistical analysis was performed with Student’s t test (B, D, F)