Fig. 1
Characteristics of human induced pluripotent stem cells (hiPSCs) cultured in different oxygen conditions. A Microscopic images of hiPSCs colonies in bright field (BF) and immunofluorescent staining for the presence of pluripotency markers: SSEA4, OCT4, and CD133. Staining of cell nuclei — DAPI. B Analysis of expression levels of transcription factors related to pluripotency: OCT4, NANOG, and SOX2 by real-time qPCR (n = 6). Cell culture conditions: N — atmospheric oxygen concentration — normoxia; H5 — hypoxia 5% O2; H3 — hypoxia 3% O2. All data are presented as the mean ± SD. Statistical significance was tested using Welch ANOVA followed by a post hoc analysis with the Dunnett test (B). *p < 0.05; ***p < 0.001