Fig. 6
Analysis of the composition and functional networks of miRNAs present in hiPS-EVs. EVs were isolated from 3 iPSC lines cultured in different oxygen conditions: N-normoxia (21% O2); H5 — hypoxia 5% O2; H3 — hypoxia 3% O2. A Percentage of the 12 most abundant miRNAs in EVs based on miRNA-seq analysis (n = 3). B Comparison of the content of individual miRNAs from the Top 12 miRNAs in hiPS-EVs (n = 3). C Heatmap of KEGG pathways for the miR-302 cluster generated by the mirPath v.3 software (DIANA Tools). WIKIPathways analysis of target genes for miR-302 cluster (D) and miRNA-gene network with depicted genes from TGFβ pathway (TGFBR2, PFN, CFN) (E) generated by the Mienturnet web tool. F Gene ontology (GO) terms (biological process, cellular component, and molecular function) for hsa-miR-302b-3p target genes created with the mirPath v.3 software. TF – transcription factor. All data are presented as the mean ± SD. Statistical significance was calculated in R using t-test (B). *p < 0.05